Submission Results

Sequence Name: Not Available

GenBank Accession Number: 1036561222

GenInfo (GI) Number: 0000000000

Download Results: 1036561222.PHASTER.zip

gi|00000000|ref|CP015026.1| Klebsiella pneumoniae strain Kpn223 plasmid pKPN-065, complete 170926, gc%: 50.58%

Download summary as .txt file: summary.txt file_download

Total: 3 prophage regions have been identified, of which 1 regions are intact, 1 regions are incomplete, and 1 regions are questionable.

RegionRegion LengthCompletenessScore# Total ProteinsRegion PositionMost Common PhageGC %Details
19.1Kbintact100114077-13177 info_outlinePHAGE_Stx2_c_1717_NC_011357(3)51.87%Show info_outline
27Kbincomplete60736407-43440 info_outlinePHAGE_Salmon_SE2_NC_016763(1)49.35%Show info_outline
327.5Kbquestionable801143527-71071 info_outlinePHAGE_Stx2_c_1717_NC_011357(3)53.26%Show info_outline
Intact (score > 90)
Questionable (score 70-90)
Incomplete (score < 70)
Region: The number assigned to the region.
Region Length: The length of the sequence of that region (in bp).
Completeness: A prediction of whether the region contains a intact or incomplete prophage based on the above criteria.
Score: The score of the region based on the above criteria.
# Total Proteins: The number of ORFs present in the region.
Region Position: The start and end positions of the region on the bacterial chromosome.
Most Common Phage: The phage(s) with the highest number of proteins most similar to those in the region.
GC %: The percentage of GC nucleotides of the region.

Criteria for scoring prophage regions (as intact, questionable, or incomplete):
Method 1:

  1. If the number of certain phage organism in this table is more than or equal to 100% of the total number of CDS of the region, the region is marked with total score 150. If less than 100%, method 2 and 3 will be used.
Method 2:
  1. If the number of certain phage organism in this table is more than 50% of the total number of CDS of the region, that phage organism is considered as the major potential phage for that region; the percentage of the total number of that phage organism in this table in the total number of proteins of the region is calculated and then multipled by 100; the percentage of the length of that phage organism in this table in the length of the region is calculated and then multipled by 50 (phage head's encapsulation capability is considered).
Method 3:
  1. If any of the specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin') are present, the score will be increased by 10 for each keyword found.
  2. If the size of the region is greater than 30 Kb, the score will be increased by 10.
  3. If there are at least 40 proteins in the region, the score will be increased by 10.
  4. If all of the phage-related proteins and hypothetical proteins constitute more than 70% of the total number of proteins in the region, the score will be increased by 10.
Compared the total score of method 2 with the total score of method 3, the bigger one is chosen as the total score of the region.
If the region's total score is less than 70, it is marked as incomplete; if between 70 to 90, it is marked as questionable; if greater than 90, it is marked as intact.

gi|00000000|ref|CP015026.1| Klebsiella pneumoniae strain Kpn223 plasmid pKPN-065, complete 170926, gc%: 50.58%

Download details as .txt file: detail.txt file_download

Hits against Virus and Prophage Database
Hits against Bacterial Database or GenBank File

Region 1, total 11 CDS

#CDS PositionBLAST HitE-ValueSequence
1complement(4077..4829)PROPHAGE_Ralsto_GMI1000: ISRSO12-transposase ORFB protein; WM91_26665; phage(gi17546158)8.48e-106Showinfo_outline
2complement(4910..5020)PROPHAGE_Ralsto_GMI1000: isrso12-transposase orfa protein; WM91_26670; phage(gi17546157)1.56e-09Showinfo_outline
35117..6133PROPHAGE_Shewan_MR-1: IS110 family transposase; WM91_26675; phage(gi24375433)2.84e-75Showinfo_outline
4complement(6322..6891)PHAGE_Entero_HK022_NC_002166: IS903 transposase; WM91_26680; phage(gi9634149)7.35e-137Showinfo_outline
5complement(6976..7311)PHAGE_Entero_HK022_NC_002166: IS903 transposase; WM91_26685; phage(gi9634149)4.27e-57Showinfo_outline
67938..8861PHAGE_Entero_HK022_NC_002166: IS903 transposase; WM91_26690; phage(gi9634149)0.0Showinfo_outline
78905..9309PHAGE_Stx2_c_1717_NC_011357: truncated transposase; WM91_26695; phage(gi209447151)5.27e-42Showinfo_outline
89306..9653PHAGE_Stx2_c_1717_NC_011357: transposase; WM91_26700; phage(gi209447152)1.81e-76Showinfo_outline
99702..11240PHAGE_Stx2_c_1717_NC_011357: transposase; WM91_26705; phage(gi209447153)0.0Showinfo_outline
10complement(11292..12230)PHAGE_Burkho_phi1026b_NC_005284: gp58; WM91_26710; phage(gi38707948)2.05e-14Showinfo_outline
11complement(12263..13177)PHAGE_Mycoba_Obama12_NC_023577: hydrolase; WM91_26715; phage(gi589891010)4.44e-09Showinfo_outline

Region 2, total 7 CDS

#CDS PositionBLAST HitE-ValueSequence
136407..37330PHAGE_Entero_HK022_NC_002166: IS903 transposase; WM91_26835; phage(gi9634149)0.0Showinfo_outline
237391..37888PHAGE_Salmon_vB_SemP_Emek_NC_018275: lysin; WM91_26840; phage(gi399498856)3.36e-99Showinfo_outline
337885..38235PHAGE_Escher_K1_dep(1)_NC_027994: putative spanin; WM91_26845; phage(gi937456982)1.51e-19Showinfo_outline
439340..40272PROPHAGE_Escher_EDL933: putative transposase; WM91_26850; phage(gi15803522)3.62e-173Showinfo_outline
540767..40988DNA gyrase subunit B; WM91_268550.0Showinfo_outline
641173..42096PHAGE_Entero_HK022_NC_002166: IS903 transposase; WM91_26860; phage(gi9634149)0.0Showinfo_outline
7complement(42415..43440)PROPHAGE_Shewan_MR-1: IS110 family transposase; WM91_26865; phage(gi24375433)4.87e-78Showinfo_outline

Region 3, total 13 CDS

#CDS PositionBLAST HitE-ValueSequence
143527..43575attL0.0Showinfo_outline
2complement(49675..49854)PHAGE_Entero_HK022_NC_002166: IS903 transposase; WM91_26875; phage(gi9634149)1.17e-33Showinfo_outline
3complement(49894..51285)PHAGE_Stx2_c_1717_NC_011357: transposase; WM91_26880; phage(gi209447153)0.0Showinfo_outline
4complement(51471..51818)PHAGE_Stx2_c_1717_NC_011357: transposase; WM91_26890; phage(gi209447152)1.32e-76Showinfo_outline
5complement(51815..52153)PHAGE_Stx2_c_1717_NC_011357: truncated transposase; WM91_26895; phage(gi209447151)5.02e-63Showinfo_outline
652204..52608PROPHAGE_Escher_CFT073: insertion element IS2 transposase InsD; WM91_26900; phage(gi26249446)7.84e-71Showinfo_outline
752909..53220PHAGE_Riemer_RAP44_NC_019490: DUF2136 superfamily protein; WM91_26905; phage(gi418489860)1.08e-11Showinfo_outline
853217..53636PHAGE_Entero_BP_4795_NC_004813: hypothetical protein; WM91_26910; phage(gi157166006)5.98e-05Showinfo_outline
953828..54751PHAGE_Entero_HK022_NC_002166: IS903 transposase; WM91_26915; phage(gi9634149)0.0Showinfo_outline
10complement(54820..55038)PROPHAGE_Xantho_33913: ISxcc1 transposase; WM91_26920; phage(gi21232565)7.16e-14Showinfo_outline
11complement(55880..57247)N-formimino-L-glutamate deiminase; WM91_269300.0Showinfo_outline
1257350..58111PHAGE_Strept_TG1_NC_018853: putative regulator, GntR family; WM91_26935; phage(gi410491962)5.08e-06Showinfo_outline
1371071..71119attR0.0Showinfo_outline
Click on a region to see details.
Intact (score > 90)
Questionable (score 70-90)
Incomplete (score < 70)

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Length: 170926 bps
Phages: 3
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Lysis
Terminase
Portal Protein
Protease
Coat Protein
Tail Shaft
Attachment Site
Integrase
Phage-like Protein
Hypotheical Protein
Other
Transposase
Fiber Protein
Plate Protein
tRNA

Download data as .txt file: png_input file_download