Submission Results

Sequence Name: Not Available

GenBank Accession Number: 1114557453

GenInfo (GI) Number: 0000000000

Download Results: 1114557453.PHASTER.zip

gi|00000000|ref|CP018434.1| Klebsiella pneumoniae strain MNCRE53 plasmid pMNCRE53_4, complete 208225, gc%: 52.88%

Download summary as .txt file: summary.txt file_download

Total: 2 prophage regions have been identified, of which 0 regions are intact, 2 regions are incomplete, and 0 regions are questionable.

RegionRegion LengthCompletenessScore# Total ProteinsRegion PositionMost Common PhageGC %Details
114.4Kbincomplete601349-14509 info_outlinePHAGE_Entero_P1_NC_005856(2)54.93%Show info_outline
234.1Kbincomplete303093597-127708 info_outlinePHAGE_Entero_P1_NC_005856(4)52.35%Show info_outline
Intact (score > 90)
Questionable (score 70-90)
Incomplete (score < 70)
Region: The number assigned to the region.
Region Length: The length of the sequence of that region (in bp).
Completeness: A prediction of whether the region contains a intact or incomplete prophage based on the above criteria.
Score: The score of the region based on the above criteria.
# Total Proteins: The number of ORFs present in the region.
Region Position: The start and end positions of the region on the bacterial chromosome.
Most Common Phage: The phage(s) with the highest number of proteins most similar to those in the region.
GC %: The percentage of GC nucleotides of the region.

Criteria for scoring prophage regions (as intact, questionable, or incomplete):
Method 1:

  1. If the number of certain phage organism in this table is more than or equal to 100% of the total number of CDS of the region, the region is marked with total score 150. If less than 100%, method 2 and 3 will be used.
Method 2:
  1. If the number of certain phage organism in this table is more than 50% of the total number of CDS of the region, that phage organism is considered as the major potential phage for that region; the percentage of the total number of that phage organism in this table in the total number of proteins of the region is calculated and then multipled by 100; the percentage of the length of that phage organism in this table in the length of the region is calculated and then multipled by 50 (phage head's encapsulation capability is considered).
Method 3:
  1. If any of the specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin') are present, the score will be increased by 10 for each keyword found.
  2. If the size of the region is greater than 30 Kb, the score will be increased by 10.
  3. If there are at least 40 proteins in the region, the score will be increased by 10.
  4. If all of the phage-related proteins and hypothetical proteins constitute more than 70% of the total number of proteins in the region, the score will be increased by 10.
Compared the total score of method 2 with the total score of method 3, the bigger one is chosen as the total score of the region.
If the region's total score is less than 70, it is marked as incomplete; if between 70 to 90, it is marked as questionable; if greater than 90, it is marked as intact.

gi|00000000|ref|CP018434.1| Klebsiella pneumoniae strain MNCRE53 plasmid pMNCRE53_4, complete 208225, gc%: 52.88%

Download details as .txt file: detail.txt file_download

Hits against Virus and Prophage Database
Hits against Bacterial Database or GenBank File

Region 1, total 15 CDS

#CDS PositionBLAST HitE-ValueSequence
149..62attL0.0Showinfo_outline
22049..2198PROPHAGE_Escher_CFT073: transposase insC; BTE50_00120; phage(gi26250372)1.30e-28Showinfo_outline
3complement(2213..2716)PHAGE_Shigel_SfIV_NC_022749: IS1 transposase B; BTE50_00125; phage(gi557307573)1.15e-118Showinfo_outline
4complement(2635..2910)PHAGE_Entero_P1_NC_005856: InsA; BTE50_00130; phage(gi46401643)7.08e-63Showinfo_outline
5complement(2944..3135)hypothetical protein; BTE50_001350.0Showinfo_outline
63189..3848PHAGE_Stx2_vB_EcoP_24B_NC_027984: Cat; BTE50_00140; phage(gi937456276)3.11e-167Showinfo_outline
7complement(4049..4426)GNAT family N-acetyltransferase; BTE50_001450.0Showinfo_outline
8complement(4493..7459)PROPHAGE_Xantho_33913: Tn5041 transposase; BTE50_00150; phage(gi21231545)8.67e-46Showinfo_outline
9complement(7462..8022)PHAGE_Sodali_phiSG1_NC_007902: resolvase; BTE50_00155; phage(gi89886020)6.53e-33Showinfo_outline
10complement(8148..8498)transposase; BTE50_001600.0Showinfo_outline
118563..8742transcriptional regulator; BTE50_001650.0Showinfo_outline
12complement(8701..9714)PHAGE_Mycoba_Butters_NC_021061: integrase; BTE50_00170; phage(gi479336499)2.26e-12Showinfo_outline
139644..9862hypothetical protein; BTE50_001750.0Showinfo_outline
149859..10356PHAGE_Bacill_Bp8p_C_NC_029121: putative dihydrofolate reductase; BTE50_00180; phage(gi985761486)1.16e-33Showinfo_outline
1514509..14522attR0.0Showinfo_outline

Region 2, total 32 CDS

#CDS PositionBLAST HitE-ValueSequence
193597..93611attL0.0Showinfo_outline
297694..98860PHAGE_Klebsi_phiKO2_NC_005857: plasmid partition protein SopA; BTE50_00740; phage(gi46402111)0.0Showinfo_outline
398860..99831PHAGE_Yersin_PY54_NC_005069: partitioning protein SpyB; BTE50_00745; phage(gi33770536)9.74e-114Showinfo_outline
499831..100793hypothetical protein; BTE50_007500.0Showinfo_outline
5complement(100820..101054)hypothetical protein; BTE50_007550.0Showinfo_outline
6101268..102466PROPHAGE_Escher_Sakai: putative transposase; BTE50_00760; phage(gi38704244)7.61e-64Showinfo_outline
7complement(102489..102704)plasmid stabilization protein; BTE50_007650.0Showinfo_outline
8complement(102783..104054)PHAGE_Cronob_ENT39118_NC_019934: DNA polymerase; BTE50_00770; phage(gi431811050)0.0Showinfo_outline
9complement(104054..104479)PHAGE_Cronob_ENT39118_NC_019934: protein umuD; BTE50_00775; phage(gi431811072)6.78e-32Showinfo_outline
10104885..106369PHAGE_Pseudo_phi3_NC_030940: hypothetical protein; BTE50_00780; phage(gi100001)1.95e-36Showinfo_outline
11106603..106833hypothetical protein; BTE50_007850.0Showinfo_outline
12107354..107779PHAGE_Pseudo_YuA_NC_010116: hypothetical protein; BTE50_00790; phage(gi162135127)1.98e-14Showinfo_outline
13108016..108270PHAGE_Escher_HK639_NC_016158: DNA pol III theta; BTE50_00795; phage(gi356870660)3.30e-22Showinfo_outline
14108305..108622hypothetical protein; BTE50_008000.0Showinfo_outline
15complement(108920..109179)hypothetical protein; BTE50_008050.0Showinfo_outline
16complement(109094..109292)hypothetical protein; BTE50_008100.0Showinfo_outline
17109404..109631hypothetical protein; BTE50_008150.0Showinfo_outline
18109723..109953hypothetical protein; BTE50_008200.0Showinfo_outline
19110005..111360hydrolase; BTE50_008250.0Showinfo_outline
20111408..111971PHAGE_Thalas_BA3_NC_009990: hypothetical protein; BTE50_00830; phage(gi160700596)2.33e-22Showinfo_outline
21complement(111866..112186)hypothetical protein; BTE50_008350.0Showinfo_outline
22complement(112305..112574)hypothetical protein; BTE50_008400.0Showinfo_outline
23112747..113289PHAGE_Salmon_SJ46_NC_031129: hypothetical protein; BTE50_00845; phage(gi100049)6.19e-64Showinfo_outline
24113338..113586transcriptional regulator; BTE50_008500.0Showinfo_outline
25113656..115692PHAGE_Gordon_Hedwig_NC_031099: RNA polymerase binding protein; BTE50_00855; phage(gi100054)5.44e-07Showinfo_outline
26115759..116190plasmid SOS inhibition protein B; BTE50_008600.0Showinfo_outline
27116187..116915plasmid SOS inhibition protein A; BTE50_008650.0Showinfo_outline
28116912..117238PHAGE_Rhodob_RcapNL_NC_020489: hypothetical protein; BTE50_00870; phage(gi461475014)4.42e-17Showinfo_outline
29117294..117668hypothetical protein; BTE50_008750.0Showinfo_outline
30complement(117657..117839)hypothetical protein; BTE50_008800.0Showinfo_outline
31complement(117846..118925)PHAGE_Spirop_SkV1_CR2_3x_NC_009987: hypothetical protein; BTE50_00885; phage(gi160688404)1.08e-08Showinfo_outline
32127708..127722attR0.0Showinfo_outline
Click on a region to see details.
Intact (score > 90)
Questionable (score 70-90)
Incomplete (score < 70)

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Length: 208225 bps
Phages: 2
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Click on a region in the genome above to show details here.
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Click on a sequence to see details.
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Lysis
Terminase
Portal Protein
Protease
Coat Protein
Tail Shaft
Attachment Site
Integrase
Phage-like Protein
Hypotheical Protein
Other
Transposase
Fiber Protein
Plate Protein
tRNA

Download data as .txt file: png_input file_download