Submission Results

Sequence Name: Wolbachia endosymbiont of Drosophila simulans, whole genome shotgun

GenBank Accession Number: AAGC00000000.1

GenInfo (GI) Number: 58537335

Download Results: AAGC00000000.1.PHASTER.zip

gi|58537335|ref|AAGC00000000.1| Wolbachia endosymbiont of Drosophila simulans gdsi_176, whole .1063100, gc%: 35.40%

Download summary as .txt file: summary.txt file_download

Total: 2 prophage regions have been identified, of which 0 regions are intact, 2 regions are incomplete, and 0 regions are questionable.

RegionRegion LengthCompletenessScore# Total ProteinsRegion PositionMost Common PhageGC %Details
124.1Kbincomplete5016860777-884959 info_outlinePHAGE_Spirop_3x35.53%Show info_outline
25.5Kbincomplete607984808-990334 info_outlinePHAGE_Clostr_phiCP26F36.78%Show info_outline
Intact (score > 90)
Questionable (score 70-90)
Incomplete (score < 70)
Region: The number assigned to the region.
Region Length: The length of the sequence of that region (in bp).
Completeness: A prediction of whether the region contains a intact or incomplete prophage based on the above criteria.
Score: The score of the region based on the above criteria.
# Total Proteins: The number of ORFs present in the region.
Region Position: The start and end positions of the region on the bacterial chromosome.
Most Common Phage: The phage(s) with the highest number of proteins most similar to those in the region.
GC %: The percentage of GC nucleotides of the region.

Criteria for scoring prophage regions (as intact, questionable, or incomplete):
Method 1:

  1. If the number of certain phage organism in this table is more than or equal to 100% of the total number of CDS of the region, the region is marked with total score 150. If less than 100%, method 2 and 3 will be used.
Method 2:
  1. If the number of certain phage organism in this table is more than 50% of the total number of CDS of the region, that phage organism is considered as the major potential phage for that region; the percentage of the total number of that phage organism in this table in the total number of proteins of the region is calculated and then multipled by 100; the percentage of the length of that phage organism in this table in the length of the region is calculated and then multipled by 50 (phage head's encapsulation capability is considered).
Method 3:
  1. If any of the specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin') are present, the score will be increased by 10 for each keyword found.
  2. If the size of the region is greater than 30 Kb, the score will be increased by 10.
  3. If there are at least 40 proteins in the region, the score will be increased by 10.
  4. If all of the phage-related proteins and hypothetical proteins constitute more than 70% of the total number of proteins in the region, the score will be increased by 10.
Compared the total score of method 2 with the total score of method 3, the bigger one is chosen as the total score of the region.
If the region's total score is less than 70, it is marked as incomplete; if between 70 to 90, it is marked as questionable; if greater than 90, it is marked as intact.

gi|58537335|ref|AAGC00000000.1| Wolbachia endosymbiont of Drosophila simulans gdsi_176, whole [asmbl_id: NC_000000].1063100, gc%: 35.40%

Download details as .txt file: detail.txt file_download

Hits against Virus and Prophage Database
Hits against Bacterial Database or GenBank File

Region 1, total 18 CDS

#CDS PositionBLAST HitE-ValueSequence
1860777..860789attL0.0Showinfo_outline
2868591..869503PHAGE_Caulob_CcrColossus: putative tRNA nucleotidyl transferase; WwSim0210; phage(gi414088345)2e-27Showinfo_outline
3complement(868825..869319)hypothetical protein; WwSim02110.0Showinfo_outline
4complement(869895..870439)conserved hypothetical protein; WwSim02660.0Showinfo_outline
5870440..8712472-oxoglutarate dehydrogenase, E1 component; WwSim06020.0Showinfo_outline
6complement(871423..872275)PHAGE_Spirop_3x: hypothetical protein SkV1VCR23x_ORF3; WwSim0666; phage(gi160688404)2e-10Showinfo_outline
7872276..873191PHAGE_Melano_entomopoxvirus: ORF MSV061 putative LINE reverse transcriptase, similar to Caenorhabditis elegans GB:U00063; WwSim0671; phage(gi9631308)1e-05Showinfo_outline
8873192..873779hypothetical protein; WwSim06800.0Showinfo_outline
9complement(874107..874609)PHAGE_Staphy_GH15: putative adsorption associated tail protein; WwSim0271; phage(gi418488029)4e-07Showinfo_outline
10complement(874606..874905)PROPHAGE_Xantho_33913: IS1480 transposase; WwSim0270_5; phage(gi21231089)4e-08Showinfo_outline
11complement(875120..875767)PHAGE_Spirop_3x: hypothetical protein SkV1VCR23x_ORF3; WwSim0670; phage(gi160688404)2e-09Showinfo_outline
12complement(875936..876719)hypothetical protein; WwSim07050.0Showinfo_outline
13878150..878663excinuclease ABC, A subunit; WwSim04050.0Showinfo_outline
14complement(879567..880146)PROPHAGE_Shewan_MR-1: ISSod13, transposase; WwSim0686; phage(gi24375047)1e-70Showinfo_outline
15complement(880467..881239)PHAGE_Spirop_3x: hypothetical protein SkV1VCR23x_ORF3; WwSim0667; phage(gi160688404)4e-09Showinfo_outline
16882266..883164conserved hypothetical protein; WwSim04150.0Showinfo_outline
17883962..883974attR0.0Showinfo_outline
18884063..884959PHAGE_Bacill_phBC6A51: Site-specific recombinase; WwSim0778; phage(gi31415812)3e-12Showinfo_outline

Region 2, total 7 CDS

#CDS PositionBLAST HitE-ValueSequence
1complement(984808..985455)PHAGE_Spirop_3x: hypothetical protein SkV1VCR23x_ORF3; WwSim0764; phage(gi160688404)2e-09Showinfo_outline
2985558..986362group II intron-associated open reading frame; WwSim07690.0Showinfo_outline
3complement(986797..987096)PROPHAGE_Xantho_33913: IS1480 transposase; WwSim0401_5; phage(gi21231089)4e-08Showinfo_outline
4complement(987226..987819)PHAGE_Pseudo_virus: virion core protein; WwSim0787; phage(gi289183845)1e-05Showinfo_outline
5complement(987970..988505)PHAGE_Aeromo_vB_AsaM_56: putative TerL large-subunit terminase; WwSim0337; phage(gi422937532)7e-20Showinfo_outline
6complement(988773..989352)PHAGE_Clostr_phiCP26F: phage tail tape measure protein; WwSim0704; phage(gi422933964)2e-06Showinfo_outline
7complement(989804..990334)PHAGE_Clostr_phiCP26F: phage tail tape measure protein; WwSim0675; phage(gi422933964)3e-05Showinfo_outline
Wolbachia endosymbiont of Drosophila simulans gdsi_176, whole
Click on a region to see details.
Intact (score > 90)
Questionable (score 70-90)
Incomplete (score < 70)

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Length: 1063100 bps
Phages: 2
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Lysis
Terminase
Portal Protein
Protease
Coat Protein
Tail Shaft
Attachment Site
Integrase
Phage-like Protein
Hypotheical Protein
Other
Transposase
Fiber Protein
Plate Protein
tRNA

Download data as .txt file: png_input file_download