Submission Results

Sequence Name: Burkholderia mallei GB8 horse 4, whole genome shotgun sequencing

GenBank Accession Number: AAHO00000000.1

GenInfo (GI) Number: 67105661

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gi|67105661|ref|AAHO00000000.1| Burkholderia mallei GB8 horse 4 , whole genome shotgun .5804858, gc%: 68.47%

Download summary as .txt file: summary.txt file_download

Total: 3 prophage regions have been identified, of which 0 regions are intact, 1 regions are incomplete, and 2 regions are questionable.

RegionRegion LengthCompletenessScore# Total ProteinsRegion PositionMost Common PhageGC %Details
18Kbincomplete5062917208-2925283 info_outlinePHAGE_Burkho_262.95%Show info_outline
220.6Kbquestionable80113126171-3146786 info_outlinePROPHAGE_Xantho_3391365.99%Show info_outline
335.6Kbquestionable80245015821-5051448 info_outlinePROPHAGE_Xantho_3391367.35%Show info_outline
Intact (score > 90)
Questionable (score 70-90)
Incomplete (score < 70)
Region: The number assigned to the region.
Region Length: The length of the sequence of that region (in bp).
Completeness: A prediction of whether the region contains a intact or incomplete prophage based on the above criteria.
Score: The score of the region based on the above criteria.
# Total Proteins: The number of ORFs present in the region.
Region Position: The start and end positions of the region on the bacterial chromosome.
Most Common Phage: The phage(s) with the highest number of proteins most similar to those in the region.
GC %: The percentage of GC nucleotides of the region.

Criteria for scoring prophage regions (as intact, questionable, or incomplete):
Method 1:

  1. If the number of certain phage organism in this table is more than or equal to 100% of the total number of CDS of the region, the region is marked with total score 150. If less than 100%, method 2 and 3 will be used.
Method 2:
  1. If the number of certain phage organism in this table is more than 50% of the total number of CDS of the region, that phage organism is considered as the major potential phage for that region; the percentage of the total number of that phage organism in this table in the total number of proteins of the region is calculated and then multipled by 100; the percentage of the length of that phage organism in this table in the length of the region is calculated and then multipled by 50 (phage head's encapsulation capability is considered).
Method 3:
  1. If any of the specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin') are present, the score will be increased by 10 for each keyword found.
  2. If the size of the region is greater than 30 Kb, the score will be increased by 10.
  3. If there are at least 40 proteins in the region, the score will be increased by 10.
  4. If all of the phage-related proteins and hypothetical proteins constitute more than 70% of the total number of proteins in the region, the score will be increased by 10.
Compared the total score of method 2 with the total score of method 3, the bigger one is chosen as the total score of the region.
If the region's total score is less than 70, it is marked as incomplete; if between 70 to 90, it is marked as questionable; if greater than 90, it is marked as intact.

gi|67105661|ref|AAHO00000000.1| Burkholderia mallei GB8 horse 4 , whole genome shotgun [asmbl_id: NC_000000].5804858, gc%: 68.47%

Download details as .txt file: detail.txt file_download

Hits against Virus and Prophage Database
Hits against Bacterial Database or GenBank File

Region 1, total 9 CDS

#CDS PositionBLAST HitE-ValueSequence
2complement(2917586..2918419)PROPHAGE_Xantho_33913: IS1477 transposase; BMAGB8_2807; phage(gi77747809)4e-88Showinfo_outline
3complement(2918443..2918706)PROPHAGE_Xantho_33913: IS1477 transposase; BMAGB8_2808; phage(gi21230912)6e-28Showinfo_outline
42919496..2919810PHAGE_Burkho_2: gp7, putative addiction module killer protein; BMAGB8_2809; phage(gi134288691)3e-54Showinfo_outline
52919813..2920169PHAGE_Burkho_2: gp6, putative addiction module antidote protein; BMAGB8_2810; phage(gi134288715)4e-60Showinfo_outline
6complement(2920213..2921268)PHAGE_Burkho_2: gp5, phage portal protein, pbsx family; BMAGB8_2811; phage(gi134288732)0.0Showinfo_outline
8complement(2921984..2922505)PROPHAGE_Escher_Sakai: ClpXP protease specificity-enhancing factor; BMAGB8_2813; phage(gi15833355)3e-24Showinfo_outline

Region 2, total 13 CDS

#CDS PositionBLAST HitE-ValueSequence
2complement(3139998..3140291)PHAGE_Ralsto_phiRSA1: transposase IRSO15-like; BMAGB8_1140; phage(gi145708108)2e-42Showinfo_outline
3complement(3140299..3141132)PROPHAGE_Xantho_33913: IS1477 transposase; BMAGB8_1141; phage(gi77747809)3e-87Showinfo_outline
4complement(3141156..3141419)PROPHAGE_Xantho_33913: IS1477 transposase; BMAGB8_1142; phage(gi21230912)6e-28Showinfo_outline
53141515..3142126PHAGE_Staphy_phiPV83: transposase; BMAGB8_1143; phage(gi9635722)9e-12Showinfo_outline
6complement(3142239..3143465)putative lipoprotein; BMAGB8_11440.0Showinfo_outline
73143622..3143753conserved hypothetical protein; BMAGB8_11450.0Showinfo_outline
83143794..3143994conserved hypothetical protein; BMAGB8_11460.0Showinfo_outline
93144060..3144323PROPHAGE_Xantho_33913: IS1477 transposase; BMAGB8_1147; phage(gi21230912)6e-28Showinfo_outline
103144347..3145180PROPHAGE_Xantho_33913: IS1477 transposase; BMAGB8_1148; phage(gi77747809)4e-88Showinfo_outline
113145202..3145525conserved hypothetical protein; BMAGB8_11490.0Showinfo_outline
123145536..3146738PHAGE_Equid__9: envelope glycoprotein J; BMAGB8_1150; phage(gi216905924)1e-04Showinfo_outline

Region 3, total 29 CDS

#CDS PositionBLAST HitE-ValueSequence
35023306..5026092PHAGE_Parame_AR158: hypothetical protein AR158_C785L; BMAGB8_A1408; phage(gi157953975)1e-42Showinfo_outline
45026168..5026911conserved hypothetical protein; BMAGB8_A14090.0Showinfo_outline
5complement(5027070..5027314)DNA-binding protein; BMAGB8_A14100.0Showinfo_outline
65027500..5028081conserved hypothetical protein; BMAGB8_02740.0Showinfo_outline
7complement(5028249..5029991)PHAGE_Pectob_My1: YadA domain-containing protein; BMAGB8_0275; phage(gi410491156)4e-10Showinfo_outline
85030122..5030385PROPHAGE_Xantho_33913: IS1477 transposase; BMAGB8_0276; phage(gi21230912)6e-28Showinfo_outline
95030409..5031242PROPHAGE_Xantho_33913: IS1477 transposase; BMAGB8_0277; phage(gi77747809)4e-88Showinfo_outline
10complement(5031253..5031861)site-specific recombinase, phage integrase family; BMAGB8_02780.0Showinfo_outline
12complement(5032124..5032267)hypothetical protein; BMAGB8_02790.0Showinfo_outline
13complement(5032264..5033886)PHAGE_Burkho_phi1026b: gp59; BMAGB8_0280; phage(gi38707949)2e-45Showinfo_outline
14complement(5034145..5035662)PHAGE_Ectoca_1: EsV-1-65; BMAGB8_0281; phage(gi13242537)5e-12Showinfo_outline
15complement(5035686..5036507)PHAGE_Ectoca_1: EsV-1-65; BMAGB8_0282; phage(gi13242537)5e-06Showinfo_outline
165036633..5037703PROPHAGE_Escher_MG1655: DNA strand exchange and recombination protein with protease and nuclease activity; BMAGB8_0283; phage(gi16130606)2e-131Showinfo_outline
175037703..5038686PHAGE_Salmon_1: putative bacteriophage tail fiber protein; Lambda gpN homolog; BMAGB8_0284; phage(gi169257204)6e-05Showinfo_outline
185038978..5039589conserved hypothetical protein; BMAGB8_02850.0Showinfo_outline
195039669..5040835succinyl-CoA synthetase beta chain; BMAGB8_02860.0Showinfo_outline
205040932..5041861succinate-CoA ligase, alpha subunit subfamily; BMAGB8_02870.0Showinfo_outline
215042012..5042725integral membrane protein, TerC family; BMAGB8_02880.0Showinfo_outline
225042893..5043462PHAGE_Deftia_14: hypothetical protein; BMAGB8_0289; phage(gi282599056)9e-05Showinfo_outline
235043700..5045487O-antigen polymerase family protein; BMAGB8_02900.0Showinfo_outline
245046026..5046499PHAGE_Human__2: EBNA-1; BMAGB8_0291; phage(gi139424506)8e-06Showinfo_outline
255046518..5046739conserved hypothetical protein; BMAGB8_02920.0Showinfo_outline
265046736..5047101TonB domain protein; BMAGB8_02930.0Showinfo_outline
28complement(5047461..5048294)PROPHAGE_Xantho_33913: IS1477 transposase; BMAGB8_0294; phage(gi77747809)4e-88Showinfo_outline
Burkholderia mallei GB8 horse 4 , whole genome shotgun
Click on a region to see details.
Intact (score > 90)
Questionable (score 70-90)
Incomplete (score < 70)

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Length: 5804858 bps
Phages: 3
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Portal Protein
Coat Protein
Tail Shaft
Attachment Site
Phage-like Protein
Hypotheical Protein
Fiber Protein
Plate Protein

Download data as .txt file: png_input file_download