Submission Results

Sequence Name: Not Available

GenBank Accession Number: 1001793595

GenInfo (GI) Number: 0000000000

Download Results: 1001793595.PHASTER.zip

gi|00000000|ref|NZ_LFPK01000006.1| Clostridium botulinum strain AM553 AM553_6, whole genome shotgun 266275, gc%: 25.71%

Download summary as .txt file: summary.txt file_download

Total: 5 prophage regions have been identified, of which 0 regions are intact, 5 regions are incomplete, and 0 regions are questionable.

RegionRegion LengthCompletenessScore# Total ProteinsRegion PositionMost Common PhageGC %Details
19.7Kbincomplete1071-9790 info_outlinePHAGE_Paenib_Harrison_NC_028746(1)26.59%Show info_outline
27.5Kbincomplete409184236-191786 info_outlinePHAGE_Brevib_Abouo_NC_029029(2)25.92%Show info_outline
314.2Kbincomplete108201262-215491 info_outlinePHAGE_Clostr_c_st_NC_007581(6)25.13%Show info_outline
46.3Kbincomplete307216515-222816 info_outlinePHAGE_Clostr_c_st_NC_007581(2)23.99%Show info_outline
58.6Kbincomplete208250179-258802 info_outlinePHAGE_Bacill_G_NC_023719(2)25.12%Show info_outline
Intact (score > 90)
Questionable (score 70-90)
Incomplete (score < 70)
Region: The number assigned to the region.
Region Length: The length of the sequence of that region (in bp).
Completeness: A prediction of whether the region contains a intact or incomplete prophage based on the above criteria.
Score: The score of the region based on the above criteria.
# Total Proteins: The number of ORFs present in the region.
Region Position: The start and end positions of the region on the bacterial chromosome.
Most Common Phage: The phage(s) with the highest number of proteins most similar to those in the region.
GC %: The percentage of GC nucleotides of the region.

Criteria for scoring prophage regions (as intact, questionable, or incomplete):
Method 1:

  1. If the number of certain phage organism in this table is more than or equal to 100% of the total number of CDS of the region, the region is marked with total score 150. If less than 100%, method 2 and 3 will be used.
Method 2:
  1. If the number of certain phage organism in this table is more than 50% of the total number of CDS of the region, that phage organism is considered as the major potential phage for that region; the percentage of the total number of that phage organism in this table in the total number of proteins of the region is calculated and then multipled by 100; the percentage of the length of that phage organism in this table in the length of the region is calculated and then multipled by 50 (phage head's encapsulation capability is considered).
Method 3:
  1. If any of the specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin') are present, the score will be increased by 10 for each keyword found.
  2. If the size of the region is greater than 30 Kb, the score will be increased by 10.
  3. If there are at least 40 proteins in the region, the score will be increased by 10.
  4. If all of the phage-related proteins and hypothetical proteins constitute more than 70% of the total number of proteins in the region, the score will be increased by 10.
Compared the total score of method 2 with the total score of method 3, the bigger one is chosen as the total score of the region.
If the region's total score is less than 70, it is marked as incomplete; if between 70 to 90, it is marked as questionable; if greater than 90, it is marked as intact.

gi|00000000|ref|NZ_LFPK01000006.1| Clostridium botulinum strain AM553 AM553_6, whole genome shotgun 266275, gc%: 25.71%

Download details as .txt file: detail.txt file_download

Hits against Virus and Prophage Database
Hits against Bacterial Database or GenBank File

Region 1, total 7 CDS

#CDS PositionBLAST HitE-ValueSequence
1complement(1..4217)PHAGE_Clostr_phi8074_B1_NC_019924: hypothetical protein; AZI61_RS11485; phage(gi431810375)5.04e-18Showinfo_outline
2complement(4461..5789)PHAGE_Entero_P1_NC_005856: Ban; AZI61_RS11490; phage(gi46401697)2.69e-77Showinfo_outline
3complement(5779..6597)PHAGE_Bacill_1_NC_009737: hypothetical protein; AZI61_RS11495; phage(gi155042921)4.74e-29Showinfo_outline
4complement(6584..7774)PHAGE_Stx2_vB_EcoP_24B_NC_027984: O; AZI61_RS11500; phage(gi937456260)1.00e-06Showinfo_outline
5complement(7816..8232)hypothetical protein; AZI61_RS115050.0Showinfo_outline
6complement(8225..8740)PHAGE_Bacill_SPO1_NC_011421: gp2.21; AZI61_RS11510; phage(gi209972885)3.63e-05Showinfo_outline
7complement(8906..9790)PHAGE_Clostr_vB_CpeS_CP51_NC_021325: putative sporulation sigma factor; AZI61_RS11515; phage(gi509140076)2.70e-05Showinfo_outline

Region 2, total 9 CDS

#CDS PositionBLAST HitE-ValueSequence
1184236..184634PHAGE_Sulfol_SMV3_NC_029103: putative transposase IS200-family protein; AZI61_RS12450; phage(gi985760792)7.26e-31Showinfo_outline
2184644..185729PHAGE_Clostr_c_st_NC_007581: putative IS transposase (OrfB); AZI61_RS12455; phage(gi80159731)0.0Showinfo_outline
3complement(186019..187086)site-specific DNA-methyltransferase; AZI61_RS124600.0Showinfo_outline
4complement(187269..188693)PHAGE_Helico_phiHP33_NC_016568: DNA primase; AZI61_RS12465; phage(gi371671350)2.65e-10Showinfo_outline
5188973..189365hypothetical protein; AZI61_RS124700.0Showinfo_outline
6189367..189579PHAGE_Bacill_1_NC_009737: predicted transcription regulator; AZI61_RS12475; phage(gi158348425)2.04e-08Showinfo_outline
7complement(189663..190316)PHAGE_Brevib_Abouo_NC_029029: transcriptional regulator; AZI61_RS12480; phage(gi971767217)5.38e-11Showinfo_outline
8complement(190412..190573)hypothetical protein; AZI61_RS124850.0Showinfo_outline
9complement(190689..191786)PHAGE_Clostr_c_st_NC_007581: putative IS transposase (OrfB); AZI61_RS12490; phage(gi80159731)0.0Showinfo_outline

Region 3, total 8 CDS

#CDS PositionBLAST HitE-ValueSequence
1complement(201262..205137)PHAGE_Clostr_c_st_NC_007581: botulinum neurotoxin type C1 precursor; AZI61_RS12560; phage(gi80159772)0.0Showinfo_outline
2complement(205163..208756)PHAGE_Clostr_c_st_NC_007581: botulinum neurotoxin type C1 nontoxic-nonhemagglutinin component; AZI61_RS12565; phage(gi80159771)0.0Showinfo_outline
3complement(208918..209454)PHAGE_Clostr_c_st_NC_007581: probable botulinum neurotoxin regulator protein; AZI61_RS12570; phage(gi80159767)2.24e-38Showinfo_outline
4209681..210559PHAGE_Clostr_c_st_NC_007581: main hemagglutinin component HA-33; AZI61_RS12575; phage(gi80159770)1.32e-41Showinfo_outline
5210622..211062PHAGE_Clostr_c_st_NC_007581: hemoagglutinin component HA-17; AZI61_RS12580; phage(gi80159769)1.77e-56Showinfo_outline
6211076..212956PHAGE_Clostr_c_st_NC_007581: hemoagglutinin component HA-70; AZI61_RS12585; phage(gi80159768)0.0Showinfo_outline
7complement(213437..214238)flagellin; AZI61_RS125950.0Showinfo_outline
8complement(214316..215491)PHAGE_Hypert_2_NC_014321: IS element Dka2 orfB; AZI61_RS12600; phage(gi300116745)2.10e-16Showinfo_outline

Region 4, total 7 CDS

#CDS PositionBLAST HitE-ValueSequence
1216515..216913PHAGE_Sulfol_SMV3_NC_029103: putative transposase IS200-family protein; AZI61_RS12615; phage(gi985760792)6.00e-36Showinfo_outline
2216924..218012PHAGE_Clostr_c_st_NC_007581: putative IS transposase (OrfB); AZI61_RS12620; phage(gi80159731)0.0Showinfo_outline
3218353..219840PHAGE_Clostr_phiCD211_NC_029048: hypothetical protein; AZI61_RS12625; phage(gi981220991)7.96e-06Showinfo_outline
4220513..221550PHAGE_Clostr_phiCD211_NC_029048: hypothetical protein; AZI61_RS12630; phage(gi981220983)2.33e-06Showinfo_outline
5complement(222024..222335)hypothetical protein; AZI61_RS126350.0Showinfo_outline
6complement(222338..222547)hypothetical protein; AZI61_RS126400.0Showinfo_outline
7complement(222568..222816)PHAGE_Clostr_phi3626_NC_003524: transcriptional regulator; AZI61_RS12645; phage(gi20066005)3.02e-18Showinfo_outline

Region 5, total 8 CDS

#CDS PositionBLAST HitE-ValueSequence
1complement(250179..250913)PHAGE_Paenib_PG1_NC_021558: hypothetical protein; AZI61_RS12850; phage(gi514231233)2.93e-09Showinfo_outline
2complement(251240..251695)hypothetical protein; AZI61_RS128550.0Showinfo_outline
3complement(251867..253099)PHAGE_Bacill_Mater_NC_027366: hypothetical protein; AZI61_RS12860; phage(gi100081)1.52e-43Showinfo_outline
4complement(253347..254210)PHAGE_Bacill_PBS1_NC_043027: 3'phosphatase, 5'polynucleotide kinase; AZI61_RS12865; phage(gi100070)5.96e-48Showinfo_outline
5complement(254478..255035)PHAGE_Bacill_G_NC_023719: gp219; AZI61_RS12870; phage(gi593777675)3.00e-28Showinfo_outline
6255392..256198PHAGE_Serrat_BF_NC_041917: hypothetical protein; AZI61_RS12875; phage(gi100349)2.03e-06Showinfo_outline
7complement(256429..256935)Flp pilus assembly complex ATPase component TadA; AZI61_RS128800.0Showinfo_outline
8complement(257078..258802)PHAGE_Phormi_MIS_PhV1B_NC_028998: protector from prophage-induced early lysis; AZI61_RS12885; phage(gi100001)1.05e-21Showinfo_outline
Click on a region to see details.
Intact (score > 90)
Questionable (score 70-90)
Incomplete (score < 70)

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Length: 266275 bps
Phages: 5
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Lysis
Terminase
Portal Protein
Protease
Coat Protein
Tail Shaft
Attachment Site
Integrase
Phage-like Protein
Hypotheical Protein
Other
Transposase
Fiber Protein
Plate Protein
tRNA

Download data as .txt file: png_input file_download