Submission Results

Sequence Name: Not Available

GenBank Accession Number: 1057477216

GenInfo (GI) Number: 0000000000

Download Results: 1057477216.PHASTER.zip

gi|00000000|ref|CP016815.1| Klebsiella pneumoniae strain ED23 plasmid unamed, complete 212770, gc%: 50.10%

Download summary as .txt file: summary.txt file_download

Total: 4 prophage regions have been identified, of which 1 regions are intact, 2 regions are incomplete, and 1 regions are questionable.

RegionRegion LengthCompletenessScore# Total ProteinsRegion PositionMost Common PhageGC %Details
17.9Kbincomplete40732163-40132 info_outlinePHAGE_Riemer_RAP44_NC_019490(1)45.01%Show info_outline
233.8Kbintact1001687984-121834 info_outlinePHAGE_Entero_fiAA91_ss_NC_022750(2)48.65%Show info_outline
310.4Kbquestionable709116728-127139 info_outlinePHAGE_Stx2_c_1717_NC_011357(3)52.17%Show info_outline
48.1Kbincomplete209165748-173925 info_outlinePHAGE_Bacill_Shanette_NC_028983(2)49.95%Show info_outline
Intact (score > 90)
Questionable (score 70-90)
Incomplete (score < 70)
Region: The number assigned to the region.
Region Length: The length of the sequence of that region (in bp).
Completeness: A prediction of whether the region contains a intact or incomplete prophage based on the above criteria.
Score: The score of the region based on the above criteria.
# Total Proteins: The number of ORFs present in the region.
Region Position: The start and end positions of the region on the bacterial chromosome.
Most Common Phage: The phage(s) with the highest number of proteins most similar to those in the region.
GC %: The percentage of GC nucleotides of the region.

Criteria for scoring prophage regions (as intact, questionable, or incomplete):
Method 1:

  1. If the number of certain phage organism in this table is more than or equal to 100% of the total number of CDS of the region, the region is marked with total score 150. If less than 100%, method 2 and 3 will be used.
Method 2:
  1. If the number of certain phage organism in this table is more than 50% of the total number of CDS of the region, that phage organism is considered as the major potential phage for that region; the percentage of the total number of that phage organism in this table in the total number of proteins of the region is calculated and then multipled by 100; the percentage of the length of that phage organism in this table in the length of the region is calculated and then multipled by 50 (phage head's encapsulation capability is considered).
Method 3:
  1. If any of the specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin') are present, the score will be increased by 10 for each keyword found.
  2. If the size of the region is greater than 30 Kb, the score will be increased by 10.
  3. If there are at least 40 proteins in the region, the score will be increased by 10.
  4. If all of the phage-related proteins and hypothetical proteins constitute more than 70% of the total number of proteins in the region, the score will be increased by 10.
Compared the total score of method 2 with the total score of method 3, the bigger one is chosen as the total score of the region.
If the region's total score is less than 70, it is marked as incomplete; if between 70 to 90, it is marked as questionable; if greater than 90, it is marked as intact.

gi|00000000|ref|CP016815.1| Klebsiella pneumoniae strain ED23 plasmid unamed, complete 212770, gc%: 50.10%

Download details as .txt file: detail.txt file_download

Hits against Virus and Prophage Database
Hits against Bacterial Database or GenBank File

Region 1, total 9 CDS

#CDS PositionBLAST HitE-ValueSequence
132163..32174attL0.0Showinfo_outline
234798..35292PHAGE_Mycoba_Adler_NC_023591: hypothetical protein; BCV49_00170; phage(gi589892236)2.02e-12Showinfo_outline
335629..36027DNA-binding protein; BCV49_001750.0Showinfo_outline
4complement(36816..37739)PHAGE_Entero_HK022_NC_002166: IS903 transposase; BCV49_00180; phage(gi9634149)0.0Showinfo_outline
5complement(37931..38350)PHAGE_Entero_BP_4795_NC_004813: hypothetical protein; BCV49_00185; phage(gi157166006)5.98e-05Showinfo_outline
6complement(38347..38658)PHAGE_Riemer_RAP44_NC_019490: DUF2136 superfamily protein; BCV49_00190; phage(gi418489860)1.08e-11Showinfo_outline
738769..38780attR0.0Showinfo_outline
8complement(38863..39351)PROPHAGE_Escher_CFT073: insertion element IS2 transposase InsD; BCV49_00195; phage(gi26249446)2.36e-92Showinfo_outline
939629..40132PHAGE_Shigel_SfIV_NC_022749: IS1 transposase B; BCV49_00200; phage(gi557307573)1.28e-118Showinfo_outline

Region 2, total 18 CDS

#CDS PositionBLAST HitE-ValueSequence
187984..87998attL0.0Showinfo_outline
2100650..101429PHAGE_Mycoba_Squirty_NC_026588: non-functional integrase; BCV49_00495; phage(gi764159894)5.56e-07Showinfo_outline
3complement(101574..102560)PROPHAGE_Escher_MG1655: predicted transposase; BCV49_00500; phage(gi16131287)5.10e-110Showinfo_outline
4complement(103024..104700)hypothetical protein; BCV49_005050.0Showinfo_outline
5105658..106524PHAGE_Brevib_Sundance_NC_028749: putative cobyrinic acid ac-diamide synthase; BCV49_00510; phage(gi971482557)1.31e-06Showinfo_outline
6106524..107555chromosome partitioning protein ParB; BCV49_005150.0Showinfo_outline
7107555..107992hypothetical protein; BCV49_005200.0Showinfo_outline
8107989..108312hypothetical protein; BCV49_005250.0Showinfo_outline
9108362..108985hypothetical protein; BCV49_005300.0Showinfo_outline
10109028..109555hypothetical protein; BCV49_005350.0Showinfo_outline
11109624..109887PROPHAGE_Xantho_33913: IS1404 transposase; BCV49_00540; phage(gi21232568)2.68e-30Showinfo_outline
12109920..110288PROPHAGE_Xantho_33913: IS1477 transposase; BCV49_00545; phage(gi77747809)3.31e-28Showinfo_outline
13complement(110339..111121)PHAGE_Entero_fiAA91_ss_NC_022750: IS21 transposition protein; BCV49_00550; phage(gi557307613)5.25e-71Showinfo_outline
14complement(111118..111693)PHAGE_Entero_fiAA91_ss_NC_022750: IS21 transposase; BCV49_00555; phage(gi557307612)1.36e-09Showinfo_outline
15complement(111757..112140)PHAGE_Entero_fiAA91_ss_NC_022750: IS21 transposase; BCV49_00560; phage(gi557307612)2.81e-11Showinfo_outline
16112563..112820PROPHAGE_Ralsto_GMI1000: isrso12-transposase orfa protein; BCV49_00565; phage(gi17546157)2.58e-41Showinfo_outline
17112909..113661PROPHAGE_Ralsto_GMI1000: ISRSO12-transposase ORFB protein; BCV49_00570; phage(gi17546158)2.78e-105Showinfo_outline
18121834..121848attR0.0Showinfo_outline

Region 3, total 9 CDS

#CDS PositionBLAST HitE-ValueSequence
1complement(116728..117738)PHAGE_Salmon_SSU5_NC_018843: putative replication protein RepA; BCV49_00585; phage(gi410491479)3.35e-116Showinfo_outline
2118468..119634PHAGE_Klebsi_phiKO2_NC_005857: plasmid partition protein SopA; BCV49_00590; phage(gi46402111)0.0Showinfo_outline
3119634..120605PHAGE_Yersin_PY54_NC_005069: partitioning protein SpyB; BCV49_00595; phage(gi33770536)5.31e-114Showinfo_outline
4122190..122630PHAGE_Stx2_c_1717_NC_011357: truncated transposase; BCV49_00600; phage(gi209447151)2.09e-12Showinfo_outline
5122627..122977PHAGE_Stx2_c_1717_NC_011357: transposase; BCV49_00605; phage(gi209447152)1.52e-49Showinfo_outline
6123008..124600PHAGE_Stx2_c_1717_NC_011357: transposase; BCV49_00610; phage(gi209447153)0.0Showinfo_outline
7complement(124928..125896)PROPHAGE_Escher_CFT073: transposase; BCV49_00615; phage(gi26248352)7.33e-10Showinfo_outline
8126139..126276PROPHAGE_Escher_CFT073: transposase insC; BCV49_00620; phage(gi26251004)2.77e-17Showinfo_outline
9126273..127139PROPHAGE_Escher_MG1655: IS2 transposase TnpB; BCV49_00625; phage(gi16132094)2.10e-178Showinfo_outline

Region 4, total 9 CDS

#CDS PositionBLAST HitE-ValueSequence
1165748..166332PHAGE_Bacill_CP_51_NC_025423: putative resistance protein; BCV49_00825; phage(gi725949538)1.72e-22Showinfo_outline
2166329..167480tellurium resistance protein TerA; BCV49_008300.0Showinfo_outline
3167503..167958PHAGE_Erwini_vB_EamM_ChrisDB_NC_031126: hypothetical protein; BCV49_00835; phage(gi100004)8.11e-08Showinfo_outline
4167982..169022PHAGE_Escher_phAPEC8_NC_020079: integral membrane protein TerC; BCV49_00840; phage(gi448260482)9.23e-97Showinfo_outline
5169061..169639PHAGE_Caulob_CcrColossus_NC_019406: putative TerD-like bacterial stress protein; BCV49_00845; phage(gi414088275)4.99e-44Showinfo_outline
6169726..170301PHAGE_Caulob_CcrColossus_NC_019406: putative TerD-like bacterial stress protein; BCV49_00850; phage(gi414088275)3.82e-40Showinfo_outline
7170386..171627PHAGE_Gordon_Cucurbita_NC_031029: hypothetical protein; BCV49_00855; phage(gi100110)7.78e-24Showinfo_outline
8complement(171964..172599)HAD family hydrolase; BCV49_008600.0Showinfo_outline
9173056..173925PHAGE_Entero_HK022_NC_002166: IS903 transposase; BCV49_00865; phage(gi9634149)0.0Showinfo_outline
Click on a region to see details.
Intact (score > 90)
Questionable (score 70-90)
Incomplete (score < 70)

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Length: 212770 bps
Phages: 4
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Lysis
Terminase
Portal Protein
Protease
Coat Protein
Tail Shaft
Attachment Site
Integrase
Phage-like Protein
Hypotheical Protein
Other
Transposase
Fiber Protein
Plate Protein
tRNA

Download data as .txt file: png_input file_download