Submission Results

Sequence Name: Not Available

GenBank Accession Number: 1142877351

GenInfo (GI) Number: 0000000000

Download Results: 1142877351.PHASTER.zip

gi|00000000|ref|CP017850.1| Klebsiella variicola strain GJ2 plasmid pKPGJ-2a, complete 108965, gc%: 55.21%

Download summary as .txt file: summary.txt file_download

Total: 2 prophage regions have been identified, of which 0 regions are intact, 2 regions are incomplete, and 0 regions are questionable.

RegionRegion LengthCompletenessScore# Total ProteinsRegion PositionMost Common PhageGC %Details
19.9Kbincomplete10135498-15428 info_outlinePHAGE_Entero_P1_NC_005856(2)57.22%Show info_outline
214.8Kbincomplete501018688-33536 info_outlinePHAGE_Entero_P1_NC_005856(2)50.57%Show info_outline
Intact (score > 90)
Questionable (score 70-90)
Incomplete (score < 70)
Region: The number assigned to the region.
Region Length: The length of the sequence of that region (in bp).
Completeness: A prediction of whether the region contains a intact or incomplete prophage based on the above criteria.
Score: The score of the region based on the above criteria.
# Total Proteins: The number of ORFs present in the region.
Region Position: The start and end positions of the region on the bacterial chromosome.
Most Common Phage: The phage(s) with the highest number of proteins most similar to those in the region.
GC %: The percentage of GC nucleotides of the region.

Criteria for scoring prophage regions (as intact, questionable, or incomplete):
Method 1:

  1. If the number of certain phage organism in this table is more than or equal to 100% of the total number of CDS of the region, the region is marked with total score 150. If less than 100%, method 2 and 3 will be used.
Method 2:
  1. If the number of certain phage organism in this table is more than 50% of the total number of CDS of the region, that phage organism is considered as the major potential phage for that region; the percentage of the total number of that phage organism in this table in the total number of proteins of the region is calculated and then multipled by 100; the percentage of the length of that phage organism in this table in the length of the region is calculated and then multipled by 50 (phage head's encapsulation capability is considered).
Method 3:
  1. If any of the specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin') are present, the score will be increased by 10 for each keyword found.
  2. If the size of the region is greater than 30 Kb, the score will be increased by 10.
  3. If there are at least 40 proteins in the region, the score will be increased by 10.
  4. If all of the phage-related proteins and hypothetical proteins constitute more than 70% of the total number of proteins in the region, the score will be increased by 10.
Compared the total score of method 2 with the total score of method 3, the bigger one is chosen as the total score of the region.
If the region's total score is less than 70, it is marked as incomplete; if between 70 to 90, it is marked as questionable; if greater than 90, it is marked as intact.

gi|00000000|ref|CP017850.1| Klebsiella variicola strain GJ2 plasmid pKPGJ-2a, complete 108965, gc%: 55.21%

Download details as .txt file: detail.txt file_download

Hits against Virus and Prophage Database
Hits against Bacterial Database or GenBank File

Region 1, total 13 CDS

#CDS PositionBLAST HitE-ValueSequence
1complement(5498..7507)PHAGE_Gordon_Schwabeltier_NC_031255: RNA polymerase binding protein; BBD64_26255; phage(gi100054)3.95e-06Showinfo_outline
2complement(7578..7796)hypothetical protein; BBD64_262600.0Showinfo_outline
3complement(8422..8739)hypothetical protein; BBD64_262650.0Showinfo_outline
4complement(8774..9028)PHAGE_Escher_HK639_NC_016158: DNA pol III theta; BBD64_26270; phage(gi356870660)3.02e-24Showinfo_outline
5complement(9221..9412)hypothetical protein; BBD64_262750.0Showinfo_outline
6complement(9455..9961)PHAGE_Rhodoc_RGL3_NC_016650: antirestriction protein; BBD64_26280; phage(gi372449791)4.61e-10Showinfo_outline
7complement(10360..11139)hypothetical protein; BBD64_262850.0Showinfo_outline
8complement(11193..11612)adenine-specific methyltransferase; BBD64_262900.0Showinfo_outline
9complement(11623..11844)PHAGE_Entero_N15_NC_001901: gp50; BBD64_26295; phage(gi9630517)2.79e-06Showinfo_outline
10complement(11844..12521)PHAGE_Staphy_Stau2_NC_030933: hypothetical protein; BBD64_26300; phage(gi100063)1.61e-27Showinfo_outline
1112881..13552PHAGE_Pseudo_F116_NC_006552: hypothetical protein; BBD64_26305; phage(gi56692976)9.87e-49Showinfo_outline
1213732..14154PHAGE_Cronob_ENT39118_NC_019934: protein umuD; BBD64_26310; phage(gi431811072)5.57e-28Showinfo_outline
1314154..15428PHAGE_Cronob_ENT39118_NC_019934: DNA polymerase; BBD64_26315; phage(gi431811050)0.0Showinfo_outline

Region 2, total 12 CDS

#CDS PositionBLAST HitE-ValueSequence
1complement(18688..19554)PHAGE_Brevib_Sundance_NC_028749: putative cobyrinic acid ac-diamide synthase; BBD64_26340; phage(gi971482557)1.46e-06Showinfo_outline
218910..18926attL0.0Showinfo_outline
320048..20680PHAGE_Clostr_vB_CpeS_CP51_NC_021325: putative resolvase; BBD64_26345; phage(gi509140089)1.26e-11Showinfo_outline
420734..20934hypothetical protein; BBD64_263500.0Showinfo_outline
521080..22009PROPHAGE_Escher_MG1655: predicted transposase; BBD64_26355; phage(gi16131287)3.70e-111Showinfo_outline
622593..23096PHAGE_Shigel_SfIV_NC_022749: IS1 transposase B; BBD64_26360; phage(gi557307573)1.20e-114Showinfo_outline
723205..24638PHAGE_Burkho_KL3_NC_015266: gp47; BBD64_26365; phage(gi327198092)4.72e-132Showinfo_outline
8complement(24672..25880)PHAGE_Burkho_KL3_NC_015266: gp45; BBD64_26370; phage(gi327198090)1.15e-40Showinfo_outline
926126..26695PHAGE_Burkho_KS14_NC_015273: gp6; BBD64_26375; phage(gi327198275)3.60e-47Showinfo_outline
1027013..27717PROPHAGE_Brucel_1330: transposase, putative; BBD64_26380; phage(gi23502708)1.98e-53Showinfo_outline
11complement(27959..30856)PROPHAGE_Xantho_33913: Tn5041 transposase; BBD64_26385; phage(gi21231545)3.03e-34Showinfo_outline
1233536..33552attR0.0Showinfo_outline
Click on a region to see details.
Intact (score > 90)
Questionable (score 70-90)
Incomplete (score < 70)

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Length: 108965 bps
Phages: 2
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Lysis
Terminase
Portal Protein
Protease
Coat Protein
Tail Shaft
Attachment Site
Integrase
Phage-like Protein
Hypotheical Protein
Other
Transposase
Fiber Protein
Plate Protein
tRNA

Download data as .txt file: png_input file_download