Submission Results

Sequence Name: Not Available

GenBank Accession Number: VHIV01000002.1

GenInfo (GI) Number: 0000000000

Download Results: VHIV01000002.1.PHASTER.zip

gi|00000000|ref|VHIV01000002.1| Bacillus sp. (in: firmicutes) strain MHSD28 Contig_2, whole genome 1474247, gc%: 35.94%

Download summary as .txt file: summary.txt file_download

Total: 2 prophage regions have been identified, of which 0 regions are intact, 2 regions are incomplete, and 0 regions are questionable.

RegionRegion LengthCompletenessScore# Total ProteinsRegion PositionMost Common PhageGC %Details
133.3Kbincomplete50271108590-1141946 info_outlinePHAGE_Bacill_PfEFR_4_NC_048641(7)35.98%Show info_outline
223.9Kbincomplete10261142056-1166026 info_outlinePHAGE_Bacill_vB_BhaS_171_NC_030904(8)35.22%Show info_outline
Intact (score > 90)
Questionable (score 70-90)
Incomplete (score < 70)
Region: The number assigned to the region.
Region Length: The length of the sequence of that region (in bp).
Completeness: A prediction of whether the region contains a intact or incomplete prophage based on the above criteria.
Score: The score of the region based on the above criteria.
# Total Proteins: The number of ORFs present in the region.
Region Position: The start and end positions of the region on the bacterial chromosome.
Most Common Phage: The phage(s) with the highest number of proteins most similar to those in the region.
GC %: The percentage of GC nucleotides of the region.

Criteria for scoring prophage regions (as intact, questionable, or incomplete):
Method 1:

  1. If the number of certain phage organism in this table is more than or equal to 100% of the total number of CDS of the region, the region is marked with total score 150. If less than 100%, method 2 and 3 will be used.
Method 2:
  1. If the number of certain phage organism in this table is more than 50% of the total number of CDS of the region, that phage organism is considered as the major potential phage for that region; the percentage of the total number of that phage organism in this table in the total number of proteins of the region is calculated and then multipled by 100; the percentage of the length of that phage organism in this table in the length of the region is calculated and then multipled by 50 (phage head's encapsulation capability is considered).
Method 3:
  1. If any of the specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin') are present, the score will be increased by 10 for each keyword found.
  2. If the size of the region is greater than 30 Kb, the score will be increased by 10.
  3. If there are at least 40 proteins in the region, the score will be increased by 10.
  4. If all of the phage-related proteins and hypothetical proteins constitute more than 70% of the total number of proteins in the region, the score will be increased by 10.
Compared the total score of method 2 with the total score of method 3, the bigger one is chosen as the total score of the region.
If the region's total score is less than 70, it is marked as incomplete; if between 70 to 90, it is marked as questionable; if greater than 90, it is marked as intact.

gi|00000000|ref|VHIV01000002.1| Bacillus sp. (in: firmicutes) strain MHSD28 Contig_2, whole genome 1474247, gc%: 35.94%

Download details as .txt file: detail.txt file_download

Hits against Virus and Prophage Database
Hits against Bacterial Database or GenBank File

Region 1, total 33 CDS

#CDS PositionBLAST HitE-ValueSequence
11108590..1108601attL0.0Showinfo_outline
21112404..1112415attL0.0Showinfo_outline
31115258..1115270attL0.0Showinfo_outline
4complement(1118582..1119547)PHAGE_Bacill_vB_BtS_BMBtp14_NC_048640: hypothetical protein; FJ659_15170; phage(gi100010)2.01e-48Showinfo_outline
51119661..1120617PHAGE_Acidia_virus_NC_029316: AAA+ ATPase; FJ659_15175; phage(gi1002166819)5.83e-05Showinfo_outline
6complement(1120886..1121509)trimeric intracellular cation channel family protein; FJ659_151800.0Showinfo_outline
71121602..1122876GTPase HflX; FJ659_151850.0Showinfo_outline
81122869..1124140aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme; FJ659_151900.0Showinfo_outline
91124317..1124706transcriptional repressor GlnR; FJ659_151950.0Showinfo_outline
101124755..1126089type I glutamate--ammonia ligase; FJ659_152000.0Showinfo_outline
111126125..1126137attR0.0Showinfo_outline
12complement(1126212..1127357)PHAGE_Bacill_phi4B1_NC_028886: site-specific recombinase; FJ659_15205; phage(gi971821285)1.86e-75Showinfo_outline
13complement(1127632..1128333)class D sortase; FJ659_152100.0Showinfo_outline
14complement(1128390..1128878)LPXTG cell wall anchor domain-containing protein; FJ659_152150.0Showinfo_outline
15complement(1129137..1129481)PHAGE_Clostr_phiCT453B_NC_029004: hypothetical protein; FJ659_15220; phage(gi100059)1.12e-10Showinfo_outline
161129966..1131141PHAGE_Bacill_vB_BtS_BMBtp14_NC_048640: hypothetical protein; FJ659_15225; phage(gi100016)1.37e-37Showinfo_outline
17complement(1131614..1131949)PHAGE_Bacill_vB_BceS_MY192_NC_048633: hypothetical protein; FJ659_15230; phage(gi100005)9.39e-14Showinfo_outline
181132191..1132400PHAGE_Bacill_Pascal_NC_027372: hypothetical protein; FJ659_15235; phage(gi100027)1.10e-09Showinfo_outline
191132453..1133205PHAGE_Bacill_PfEFR_4_NC_048641: endolysin; FJ659_15240; phage(gi100033)2.01e-125Showinfo_outline
201133237..1133497PHAGE_Bacill_PfEFR_4_NC_048641: holin; FJ659_15245; phage(gi100034)1.99e-46Showinfo_outline
211133885..1134619PHAGE_Bacill_PfEFR_4_NC_048641: putative phosphoesterase; FJ659_15250; phage(gi100037)2.07e-112Showinfo_outline
221134567..1135430PHAGE_Lister_B054_NC_009813: gp53; FJ659_15255; phage(gi157325337)2.23e-43Showinfo_outline
231135801..1135812attR0.0Showinfo_outline
241135957..1136151PHAGE_Bacill_vB_BceS_MY192_NC_048633: hypothetical protein; FJ659_15260; phage(gi100012)3.24e-33Showinfo_outline
251136168..1136446PHAGE_Bacill_vB_BtS_BMBtp14_NC_048640: hypothetical protein; FJ659_15265; phage(gi100024)2.36e-16Showinfo_outline
261136439..1136798PHAGE_Bacill_vB_BtS_BMBtp14_NC_048640: hypothetical protein; FJ659_15270; phage(gi100025)4.19e-42Showinfo_outline
271136817..1136984PHAGE_Bacill_PfEFR_4_NC_048641: hypothetical protein; FJ659_15275; phage(gi100040)3.66e-18Showinfo_outline
281137010..1137261PHAGE_Bacill_PfEFR_4_NC_048641: hypothetical protein; FJ659_15280; phage(gi100042)4.15e-22Showinfo_outline
291137281..1137790PHAGE_Aeriba_AP45_NC_048651: hypothetical protein; FJ659_15285; phage(gi100019)1.54e-36Showinfo_outline
30complement(1138254..1138640)spore coat protein; FJ659_152900.0Showinfo_outline
311140515..1140997PHAGE_Bacill_phIS3501_NC_019502: ArpU family phage transcriptional regulator; FJ659_15295; phage(gi422934318)3.43e-99Showinfo_outline
321140997..1141539PHAGE_Bacill_PfEFR_4_NC_048641: hypothetical protein; FJ659_15300; phage(gi100061)7.19e-65Showinfo_outline
331141946..1141957attR0.0Showinfo_outline

Region 2, total 26 CDS

#CDS PositionBLAST HitE-ValueSequence
1complement(1142056..1142256)PHAGE_Vibrio_vB_VspP_pVa5_NC_049379: hypothetical protein; FJ659_15305; phage(gi100064)8.52e-10Showinfo_outline
21142850..1143371hypothetical protein; FJ659_153100.0Showinfo_outline
31143804..1144472hypothetical protein; FJ659_153150.0Showinfo_outline
41144532..1144750PHAGE_Bacill_BceA1_NC_048628: hypothetical protein; FJ659_15320; phage(gi100002)2.26e-35Showinfo_outline
51144757..1145161PHAGE_Bacill_WBeta_NC_007734: hypothetical protein; FJ659_15325; phage(gi85701427)2.00e-60Showinfo_outline
61145158..1145493PHAGE_Bacill_vB_BhaS_171_NC_030904: hypothetical protein; FJ659_15330; phage(gi100004)3.40e-41Showinfo_outline
71145617..1145928PHAGE_Bacill_vB_BhaS_171_NC_030904: hypothetical protein; FJ659_15335; phage(gi100005)8.06e-25Showinfo_outline
81145925..1147592PHAGE_Bacill_vB_BhaS_171_NC_030904: hypothetical protein; FJ659_15340; phage(gi100006)0.0Showinfo_outline
91147601..1148746PHAGE_Bacill_vB_BhaS_171_NC_030904: hypothetical protein; FJ659_15345; phage(gi100007)0.0Showinfo_outline
101148746..1149489PHAGE_Bacill_vB_BhaS_171_NC_030904: hypothetical protein; FJ659_15350; phage(gi100008)5.84e-39Showinfo_outline
111149493..1150647PHAGE_Bacill_vB_BhaS_171_NC_030904: hypothetical protein; FJ659_15355; phage(gi100009)8.48e-124Showinfo_outline
121150653..1150946PHAGE_Bacill_vB_BhaS_171_NC_030904: hypothetical protein; FJ659_15360; phage(gi100011)6.95e-44Showinfo_outline
131150948..1151301PHAGE_Bacill_vB_BhaS_171_NC_030904: hypothetical protein; FJ659_15365; phage(gi100012)8.32e-30Showinfo_outline
141151303..1151644PHAGE_Strept_phiARI0131_2_NC_031941: hypothetical protein; FJ659_15370; phage(gi100011)5.86e-19Showinfo_outline
151151644..1151973PHAGE_Bacill_1_NC_009737: putative aminopeptidase; FJ659_15375; phage(gi155042942)9.66e-30Showinfo_outline
161151974..1152561PHAGE_Strept_phiARI0131_2_NC_031941: hypothetical protein; FJ659_15380; phage(gi100009)1.02e-50Showinfo_outline
171152568..1152924PHAGE_Strept_phiARI0131_2_NC_031941: hypothetical protein; FJ659_15385; phage(gi100008)1.85e-10Showinfo_outline
181153155..1154363PHAGE_Strept_phiARI0131_2_NC_031941: hypothetical protein; FJ659_15390; phage(gi100007)8.99e-31Showinfo_outline
191154621..1154878PHAGE_Clostr_phiCD111_NC_028905: Tail tape measure; FJ659_15395; phage(gi971820061)1.16e-06Showinfo_outline
201155006..1155785PHAGE_Bacill_Staley_NC_022767: HNH homing endonuclease III; FJ659_15400; phage(gi568191180)5.39e-31Showinfo_outline
211156017..1158194PHAGE_Paenib_PBL1c_NC_048689: hypothetical protein; FJ659_15405; phage(gi100014)9.28e-127Showinfo_outline
221158236..1159726PHAGE_Bacill_PfEFR_4_NC_048641: hypothetical protein; FJ659_15410; phage(gi100018)0.0Showinfo_outline
231159723..1164399PHAGE_Bacill_vB_BtS_B83_NC_048762: hypothetical protein; FJ659_15415; phage(gi100022)0.0Showinfo_outline
241164490..1164726PHAGE_Bacill_phBC6A51_NC_004820: XpaF1 protein; FJ659_15420; phage(gi31415801)1.45e-39Showinfo_outline
251164726..1164965PHAGE_Paenib_Likha_NC_048693: hypothetical protein; FJ659_15425; phage(gi100022)2.80e-12Showinfo_outline
261164962..1166026PHAGE_Bacill_phBC6A51_NC_004820: N-acetylmuramoyl-L-alanine amidase; FJ659_15430; phage(gi31415803)0.0Showinfo_outline
Bacillus sp
Click on a region to see details.
Intact (score > 90)
Questionable (score 70-90)
Incomplete (score < 70)

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Length: 1474247 bps
Phages: 2
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Lysis
Terminase
Portal Protein
Protease
Coat Protein
Tail Shaft
Attachment Site
Integrase
Phage-like Protein
Hypotheical Protein
Other
Transposase
Fiber Protein
Plate Protein
tRNA

Download data as .txt file: png_input file_download