Submission Results

Sequence Name: Not Available

GenBank Accession Number: WJDP01000021.1

GenInfo (GI) Number: 0000000000

Download Results: WJDP01000021.1.PHASTER.zip

gi|00000000|ref|WJDP01000021.1| Bacillus thuringiensis strain 4AZ3 68681, gc%: 34.13%

Download summary as .txt file: summary.txt file_download

Total: 2 prophage regions have been identified, of which 1 regions are intact, 1 regions are incomplete, and 0 regions are questionable.

RegionRegion LengthCompletenessScore# Total ProteinsRegion PositionMost Common PhageGC %Details
111.3Kbincomplete20191-11397 info_outlinePHAGE_Bacill_phiCM3_NC_023599(4)35.28%Show info_outline
218.2Kbintact1001850480-68681 info_outlinePHAGE_Bacill_phi4J1_NC_029008(13)37.19%Show info_outline
Intact (score > 90)
Questionable (score 70-90)
Incomplete (score < 70)
Region: The number assigned to the region.
Region Length: The length of the sequence of that region (in bp).
Completeness: A prediction of whether the region contains a intact or incomplete prophage based on the above criteria.
Score: The score of the region based on the above criteria.
# Total Proteins: The number of ORFs present in the region.
Region Position: The start and end positions of the region on the bacterial chromosome.
Most Common Phage: The phage(s) with the highest number of proteins most similar to those in the region.
GC %: The percentage of GC nucleotides of the region.

Criteria for scoring prophage regions (as intact, questionable, or incomplete):
Method 1:

  1. If the number of certain phage organism in this table is more than or equal to 100% of the total number of CDS of the region, the region is marked with total score 150. If less than 100%, method 2 and 3 will be used.
Method 2:
  1. If the number of certain phage organism in this table is more than 50% of the total number of CDS of the region, that phage organism is considered as the major potential phage for that region; the percentage of the total number of that phage organism in this table in the total number of proteins of the region is calculated and then multipled by 100; the percentage of the length of that phage organism in this table in the length of the region is calculated and then multipled by 50 (phage head's encapsulation capability is considered).
Method 3:
  1. If any of the specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin') are present, the score will be increased by 10 for each keyword found.
  2. If the size of the region is greater than 30 Kb, the score will be increased by 10.
  3. If there are at least 40 proteins in the region, the score will be increased by 10.
  4. If all of the phage-related proteins and hypothetical proteins constitute more than 70% of the total number of proteins in the region, the score will be increased by 10.
Compared the total score of method 2 with the total score of method 3, the bigger one is chosen as the total score of the region.
If the region's total score is less than 70, it is marked as incomplete; if between 70 to 90, it is marked as questionable; if greater than 90, it is marked as intact.

gi|00000000|ref|WJDP01000021.1| Bacillus thuringiensis strain 4AZ3 68681, gc%: 34.13%

Download details as .txt file: detail.txt file_download

Hits against Virus and Prophage Database
Hits against Bacterial Database or GenBank File

Region 1, total 19 CDS

#CDS PositionBLAST HitE-ValueSequence
11..227PHAGE_Bacill_vB_BtS_B83_NC_048762: hypothetical protein; GH855_24140; phage(gi100023)3.32e-32Showinfo_outline
2265..549PHAGE_Aeriba_AP45_NC_048651: hypothetical protein; GH855_24145; phage(gi100065)1.45e-15Showinfo_outline
3564..794PHAGE_Bacill_Waukesha92_NC_025424: SPP1 family holin; GH855_24150; phage(gi712912404)1.51e-44Showinfo_outline
4811..1527PHAGE_Bacill_phiCM3_NC_023599: N-acetylmuramoyl-L-alanine amidase; GH855_24155; phage(gi589892730)2.87e-93Showinfo_outline
5complement(1591..2073)protein phosphatase 2C; GH855_241600.0Showinfo_outline
6complement(2194..2535)PHAGE_Staphy_phiRS7_NC_022914: YolD-like protein; GH855_24165; phage(gi560185985)5.05e-15Showinfo_outline
7complement(2532..3797)PHAGE_Bacill_SPbeta_NC_001884: IMPB/MUCB/SAMB family protein; GH855_24170; phage(gi9630142)9.51e-137Showinfo_outline
8complement(3921..4208)hypothetical protein; GH855_241750.0Showinfo_outline
9complement(4313..4537)PHAGE_Bacill_Waukesha92_NC_025424: DNA binding protein; GH855_24180; phage(gi712912401)5.45e-25Showinfo_outline
104851..5045PHAGE_Bacill_phiCM3_NC_023599: hypothetical protein; GH855_24185; phage(gi589892733)8.58e-19Showinfo_outline
115058..6224PHAGE_Bacill_phiCM3_NC_023599: cell division FtsK/SpoIIIE protein; GH855_24190; phage(gi589892734)0.0Showinfo_outline
126214..6822PHAGE_Bacill_phiCM3_NC_023599: hypothetical protein; GH855_24195; phage(gi589892735)1.05e-113Showinfo_outline
137057..7251hypothetical protein; GH855_242000.0Showinfo_outline
147342..7932PHAGE_Bacill_Deep_Purple_NC_048667: hypothetical protein; GH855_24205; phage(gi100007)3.82e-20Showinfo_outline
158041..8541PHAGE_Erwini_vB_EamM_Asesino_NC_031107: hypothetical protein; GH855_24210; phage(gi100099)2.35e-08Showinfo_outline
16complement(8849..9466)nucleotidyltransferase domain-containing protein; GH855_242150.0Showinfo_outline
17complement(9835..10119)hypothetical protein; GH855_242200.0Showinfo_outline
18complement(10151..10552)hypothetical protein; GH855_242250.0Showinfo_outline
19complement(10567..11397)PHAGE_Lactob_Lb_NC_047983: minor tail protein; GH855_24230; phage(gi100055)9.56e-47Showinfo_outline

Region 2, total 18 CDS

#CDS PositionBLAST HitE-ValueSequence
150480..51055PHAGE_Burkho_KS14_NC_015273: gp6; GH855_24465; phage(gi327198275)4.15e-39Showinfo_outline
251189..51503hypothetical protein; GH855_244700.0Showinfo_outline
352233..52991PHAGE_Bacill_250_NC_029024: phage-related terminase small subunit-like protein; GH855_24475; phage(gi971766634)3.48e-87Showinfo_outline
452988..54217PHAGE_Staphy_DW2_NC_024391: terminase large subunit; GH855_24480; phage(gi658608067)0.0Showinfo_outline
554230..55654PHAGE_Staphy_SpaA1_NC_018277: phage portal protein, SPP1; GH855_24485; phage(gi399498867)0.0Showinfo_outline
655728..56495PHAGE_Staphy_SpaA1_NC_018277: phage minor head protein; GH855_24490; phage(gi399498868)0.0Showinfo_outline
756570..57301PHAGE_Staphy_SpaA1_NC_018277: phage scaffold protein; GH855_24495; phage(gi399498870)2.78e-106Showinfo_outline
857418..58263PHAGE_Staphy_SpaA1_NC_018277: phage coat protein gp6; GH855_24500; phage(gi399498871)0.0Showinfo_outline
958306..58617PHAGE_Staphy_SpaA1_NC_018277: phage related protein gp15; GH855_24505; phage(gi399498872)5.46e-54Showinfo_outline
1058614..58958PHAGE_Staphy_SpaA1_NC_018277: phage head-tail adaptor; GH855_24510; phage(gi399498873)1.71e-73Showinfo_outline
1158933..59340PHAGE_Staphy_SpaA1_NC_018277: phage protein, HK97 gp10; GH855_24515; phage(gi399498874)4.23e-90Showinfo_outline
1259346..59708PHAGE_Staphy_SpaA1_NC_018277: phage structural protein; GH855_24520; phage(gi399498875)3.31e-75Showinfo_outline
1359723..60316PHAGE_Staphy_SpaA1_NC_018277: phage related protein; GH855_24525; phage(gi399498876)2.22e-136Showinfo_outline
1460363..60791PHAGE_Staphy_SpaA1_NC_018277: phage related protein; GH855_24530; phage(gi399498877)1.86e-82Showinfo_outline
1560896..61102PHAGE_Staphy_SpaA1_NC_018277: phage related protein; GH855_24535; phage(gi399498878)5.74e-42Showinfo_outline
1661103..64042PHAGE_Staphy_SpaA1_NC_018277: phage tail tape measure protein; GH855_24540; phage(gi399498879)0.0Showinfo_outline
1764055..65527PHAGE_Staphy_SpaA1_NC_018277: phage tail fiber protein; GH855_24545; phage(gi399498880)0.0Showinfo_outline
1865524..68681PHAGE_Bacill_vB_BceS_MY192_NC_048633: tail assembly protein; GH855_24550; phage(gi100053)0.0Showinfo_outline
Click on a region to see details.
Intact (score > 90)
Questionable (score 70-90)
Incomplete (score < 70)

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Length: 68681 bps
Phages: 2
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Lysis
Terminase
Portal Protein
Protease
Coat Protein
Tail Shaft
Attachment Site
Integrase
Phage-like Protein
Hypotheical Protein
Other
Transposase
Fiber Protein
Plate Protein
tRNA

Download data as .txt file: png_input file_download